N. Takahashi et al., A NEW SCREENING METHOD FOR THE SELECTION OF LACTOBACILLUS-ACIDOPHILUSGROUP LACTIC-ACID BACTERIA WITH HIGH ADHESION TO HUMAN COLONIC MUCOSA, Bioscience, biotechnology, and biochemistry, 60(9), 1996, pp. 1434-1438
A hemagglutination (HA) assay was done for the screening of lectin-lik
e components in surface layer protein (SLP) from Lactobacillus (L.) ac
idophilus A group strains, The new screening method, using polystyrene
beads coated with rat-colonic mucin (RCM), which combines sugar chain
s similar to those of human colonic mucin, was also done, The results
showed that the HA assay was not a good indicator for selecting strain
s having high adhesion to the human intestinal tract, The SLPs from 3
strains that strongly bound to RCM also bound web to carbohydrate port
ions of Carnoy's-fixed human colonic mucous layer, These results sugge
st that this method is a new promising screening technique for the L.
acidophilus strains having high adhesion to the human intestinal tract
.