EXPRESSION OF 135-KDA INSECTICIDAL PROTEIN GENE FROM BACILLUS-THURINGIENSIS IN THE YEAST SACCHAROMYCES-CEREVISIAE

Authors
WATANABE K TOYODA M HASHIMOTO H NAKAGAWA K NAKAMURA K YABUSAKI Y TANIMURA A SAITO Y
Citation
K. Watanabe et al., EXPRESSION OF 135-KDA INSECTICIDAL PROTEIN GENE FROM BACILLUS-THURINGIENSIS IN THE YEAST SACCHAROMYCES-CEREVISIAE, Bioscience, biotechnology, and biochemistry, 60(9), 1996, pp. 1483-1485
Citations number
14
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
60
Issue
9
Year of publication
1996
Pages
1483 - 1485
Database
ISI
SICI code
0916-8451(1996)60:9<1483:EO1IPG>2.0.ZU;2-O
Abstract
Bacillus thuringiensis subsp, aizawai produces 130-kDa and 135-kDa (Cr yIA(a)) insecticidal proteins. When Saccharomyces cerevisiae was trans formed by the vector carrying a cryIA(a) gene, the gene expression cou ld not be observed, When the 5'-upstream region from the initiation co don was removed using a synthetic oligonucleotide, the CryIA(a) protei n was successfully synthesized in yeast, The yeast extract containing CryIA(a) protein had insecticidal activity against Plutella xylostella larvae.