CLEAVAGE SPECIFICITY OF COXSACKIEVIRUS 3C PROTEINASE FOR PEPTIDE SUBSTRATE-(2) - IMPORTANCE OF THE P2 AND P4 RESIDUES

Authors
MIYASHITA K OKUNISHI J UTSUMI R TAGIRI S HOTTA K KOMANO T TAMURA T SATOH N
Citation
K. Miyashita et al., CLEAVAGE SPECIFICITY OF COXSACKIEVIRUS 3C PROTEINASE FOR PEPTIDE SUBSTRATE-(2) - IMPORTANCE OF THE P2 AND P4 RESIDUES, Bioscience, biotechnology, and biochemistry, 60(9), 1996, pp. 1528-1529
Citations number
14
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
60
Issue
9
Year of publication
1996
Pages
1528 - 1529
Database
ISI
SICI code
0916-8451(1996)60:9<1528:CSOC3P>2.0.ZU;2-G
Abstract
Coxsackievirus 3C proteinase (3C(pro)) cleaves between Gin and Gly, bu t additional amino acids are required to constitute a cleavage site, T o investigate the additional sequence requirements, cleavages of the p eptide substrate, and its derivatives were examined, Substitutions of each residue from the P2 to P5 positions showed the importance of the P2 Phe and P4 Ala for recognition by 3C(pro).