AN ADDITIONAL IONIC BOND SUGGESTED BY MOLECULAR MODELING OF TEM-2 MIGHT INDUCE A SLIGHT DISCREPANCY BETWEEN CATALYTIC PROPERTIES OF TEM-1 AND TEM-2 BETA-LACTAMASES

Authors
CHAIBI EB FARZANEH S PEDUZZI J BARTHELEMY M LABIA R
Citation
Eb. Chaibi et al., AN ADDITIONAL IONIC BOND SUGGESTED BY MOLECULAR MODELING OF TEM-2 MIGHT INDUCE A SLIGHT DISCREPANCY BETWEEN CATALYTIC PROPERTIES OF TEM-1 AND TEM-2 BETA-LACTAMASES, FEMS microbiology letters, 143(2-3), 1996, pp. 121-125
Citations number
21
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
143
Issue
2-3
Year of publication
1996
Pages
121 - 125
Database
ISI
SICI code
0378-1097(1996)143:2-3<121:AAIBSB>2.0.ZU;2-3
Abstract
The plasmid-mediated TEM-1 and TEM-2 beta-lactamases are the most comm only encountered among Gram-negative bacteria. They belong to molecula r class A, and differ by one amino acid at position 39: TEM-1 have a g lutamine and TEM-2 a lysine. Kinetic parameters (k(cat) and K-m) and c atalytic efficiency (k(cat)/K-m) of TEM-1 and TEM-2 beta-lactamases ar e slightly, but significantly different. For all antibiotics except me thicillin and cefazolin, the catalytic efficiency values of TEM-2 are clearly greater than that of TEM-1. Molecular modelling of TEM-2, when compared to that of TEM-1, showed an additional ionic bond between Ly s-39 and Glu-281.