CARBOXYL-TERMINAL REGION OF THE MUKB PROTEIN IN ESCHERICHIA-COLI IS ESSENTIAL FOR DNA-BINDING ACTIVITY

Authors
SALEH AZM YAMANAKA K NIKI H OGURA T YAMAZOE M HIRAGA S
Citation
Azm. Saleh et al., CARBOXYL-TERMINAL REGION OF THE MUKB PROTEIN IN ESCHERICHIA-COLI IS ESSENTIAL FOR DNA-BINDING ACTIVITY, FEMS microbiology letters, 143(2-3), 1996, pp. 211-216
Citations number
12
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
143
Issue
2-3
Year of publication
1996
Pages
211 - 216
Database
ISI
SICI code
0378-1097(1996)143:2-3<211:CROTMP>2.0.ZU;2-4
Abstract
The purified MukB protein of Escherichia coli has DNA binding activity and nucleotide binding activity. We have isolated a mutation, mukB101 3, causing a substitution of valine at position 1379 to leucine. This mutant MukB protein was defective for DNA binding, while the ATP bindi ng activity remained unaffected. A truncated MukB protein that is shor t of 109 amino acids from the C-terminus failed to bind DNA.