INHIBITORY EFFECT OF THIOPHOSPHORIC ACID ALKALOID DERIVATIVES FROM CHELIDONIUM-MAJUS L (UKRAIN) ON OVALBUMIN ANTIGENICITY AND ANTIOVALBUMINIGE ANTIBODY-RESPONSE IN MICE

The ability of the Chelidonium majus L. alkaloid derivative Ukrain (UK ) to inhibit ovalbumin-induced sensitization was tested in BALB/c and F1(BALB/c x C57BL/6J) mice. UK introduced into the mice in the mixture with antigen (ovalbumin) and adjuvant (alum) inhibited the sensitizat ion of mice, reflected in lower anti-GA IgE antibody response and decr eased antigen-induced histamine release from mast cells isolated from peritoneal cavities of sensitized mice. The effect of UK on the antige nicity of ovalbumin (OA) in anaphylaxis was tested in heterologous pas sive cutaneous anaphylactic (PCA) reaction on rats. The results show t hat the OA prepared in the mixture with UK had a decreased ability to react with anti-GA IgE antibodies raised against native OA in mice and fixed on the surface of rat mast cells in heterologous PCA reactions. The results suggest that UK pretreatment of OA may affect its antigen ic property and the ability to react with anti-GA IgE antibodies raise d against the native IgE molecules.