INHIBITION OF CHEMILUMINESCENCE RESPONSE OF HUMAN MONONUCLEAR-CELLS AND SUPPRESSION OF MITOGEN-INDUCED PROLIFERATION OF SPLEEN LYMPHOCYTES OF MICE BY HISPOLON AND HISPIDIN

The effects of two phenolic compounds, hispolon and hispidin isolated from fruit bodies of the basidiomycete Inonotus hispidus (Bull. ex Pr. ) Karst, were investigated on the chemiluminescence response by LPS- o r zymosan-activated human mononuclear cells (MNC) and on the concanava lin A-induced proliferation of spleen lymphocytes of mouse in vitro. B oth compounds showed inhibitory activity in the chemiluminescence-test with an IC(5)0 (the concentration of test compound causing 50% effect ) ranging from 4.4 to 4.6 mu g/ml (20.3 to 21.2 mu M) for hispolon and < 0.1 to 1.5 mu g/ml (from < 0.4 mu M to 6.0 mu M) for hispidin. Anti proliferative effects have been achieved in the lymphocyte transformat ion test (LTT) by hispolon with an IC50 of 3.4 mu g/ml (15.5 mu M).