CLONING (CAG GTC)N STSS BY AN ALU-(CAG/GTC)N PCR METHOD - AN APPROACHTO HUMAN-CHROMOSOME-12 AND SPINOCEREBELLAR ATAXIA-2 (SCA2)/

Authors
PUJANA MA VOLPINI V ESTIVILL X
Citation
Ma. Pujana et al., CLONING (CAG GTC)N STSS BY AN ALU-(CAG/GTC)N PCR METHOD - AN APPROACHTO HUMAN-CHROMOSOME-12 AND SPINOCEREBELLAR ATAXIA-2 (SCA2)/, Nucleic acids research, 24(18), 1996, pp. 3651-3652
Citations number
19
Categorie Soggetti
Biology
Journal title
Nucleic acids research → ACNP
ISSN journal
03051048
Volume
24
Issue
18
Year of publication
1996
Pages
3651 - 3652
Database
ISI
SICI code
0305-1048(1996)24:18<3651:C(GSBA>2.0.ZU;2-4
Abstract
We report here an Alu-(CAG/GTC)n PCR method for the cloning of STSs wi th (CAG/GTC)n sequences. We have applied this method to genomic DNA of a somatic cell hybrid containing human chromosome 12 where linkage ha s been found for a known familiar dominant ataxia (SCA2), which is tho ught to be due to a (CAG/GTC)n expansion. We have isolated several clo nes containing (CAG/GTC)n sequences, which include previously identifi ed sequences that map to chromosome 12. This method could be a new PCR approach for the cloning of repeats based on their proximity to Alu s equences.