INHIBITION OF LIPID-PEROXIDATION AND THE ACTIVE OXYGEN RADICAL SCAVENGING EFFECT OF ANTHOCYANIN PIGMENTS ISOLATED FROM PHASEOLUS-VULGARIS L

No attention has been paid to anthocyanin pigments from the viewpoint of inhibitors of lipid peroxidation and scavengers of active oxygen ra dicals; therefore, we investigated the antioxidative, radical scavengi ng, and inhibitory effects on lipid peroxidation by UV light irradiati on of three anthocyanin pigments, pelargonidin 3-O-beta-D-glucoside (P 3G), cyanidin 3-O-beta-D-glucoside (C3G), and delphinidin 3-O-beta-D-g lucoside (D3G), isolated from the Phaseolus vulgaris L. seed coat, and their aglycons, pelargonidin chloride (Pel), cyanidin chloride (Cy), and delphinidin chloride (Del). All pigments had strong antioxidative activity in a liposomal system and reduced the formation of malondiald ehyde by UVB irradiation. On the other hand, the extent of antioxidati ve activity in a rat liver microsomal system and the scavenging effect of hydroxyl radicals (. OH) and superoxide anion radicals (O-2(-)) we re influenced by their own structures.