CLONING AND STUDYING THE EXPRESSION OF TH E CIP1 GENE OF PSEUDOMONAS-AERUGINOSA TRANSPOSABLE PHAGE D3112 IN THE HOMOLOGOUS HOST AND IN ESCHERICHIA-COLI

Regulatory gene cip1 of Pseudomonas aeruginosa transposable phage D311 2 was cloned, and its expression was studied in P. aeruginosa and Esch erichia coli. Overexpression of the cip1 gene prevents transcription a cid replication of phage D3112 DNA and also lysogenization of bacteria P. aeruginosa PAO1 by phage D3112. The direction of cip1 gene transcr iption within the vector was determined in the study of cip1 gene expr ession, dependent on its orientation toward the gene lacZ promoter. Th e expression of the cloned cip1 gene inhibited the specific TCS phenot ype of E. coli (RP4 :: D3112) cells. The functional homology of the ci p1 gene of phage D3112 and the negative regulator ner of E. coli Mu1 p hage was discussed.