INTERACTION OF HUMAN LEUKOTRIENE C-4 SYNTHASE AND MICROSOMAL GLUTATHIONE TRANSFERASE IN-VIVO

Microsomal glutathione transferase (mGT) specifically binds leukotrien e C-4 synthase in the presence of Mg2+ ion (Soderstrom et al., Protein Expression and Purification (1995) 6, 352-356). To investigate if thi s interaction occurs in vivo we screened a human lung cDNA library wit h a bait vector encoding human mGT in the yeast two-hybrid system. One of the five positive clones obtained encoded leukotriene C, synthase. This clone was expressed in two heterologous systems. The recombinant protein cross-reacted with a guinea pig antibody raised against a Key hole limper hemocyanin coupled synthetic peptide corresponding to amin o acids 141-150 of human leukotriene C-4 synthase. (C) 1996 Academic P ress, Inc.