INTERACTION OF BOTH THE C2A AND C2B DOMAINS OF RABPHILIN3 WITH CA2+ AND PHOSPHOLIPID

Authors
OISHI H SASAKI T TAKAI Y
Citation
H. Oishi et al., INTERACTION OF BOTH THE C2A AND C2B DOMAINS OF RABPHILIN3 WITH CA2+ AND PHOSPHOLIPID, Biochemical and biophysical research communications, 229(2), 1996, pp. 498-503
Citations number
21
Categorie Soggetti
Biology,Biophysics
Journal title
Biochemical and biophysical research communications → ACNP
ISSN journal
0006291X
Volume
229
Issue
2
Year of publication
1996
Pages
498 - 503
Database
ISI
SICI code
0006-291X(1996)229:2<498:IOBTCA>2.0.ZU;2-6
Abstract
Rabphilin3, a downstream target molecule of the Rab3 subfamily small G proteins, has two C2 domains (the C2A and C2B domains) at its C-termi nal region and is implicated in Ca2+-dependent neurotransmitter releas e. Rabphilin3 interacts with Ca2+ and phospholipid at its C2 domain, b ut it remains to be clarified which domain, the C2A or C2B domain, int eracts with these compounds. We have found here that both the C2A and C2B domains interact with Ca2+ with similar kinetics but interact with phospholipid with slightly different kinetics. (C) 1996 Academic Pres s, Inc.