A MICROFABRICATED, ELECTROCHEMILUMINESCENCE CELL FOR THE DETECTION OFAMPLIFIED DNA

Authors
HSUEH YT SMITH RL NORTHRUP MA
Citation
Yt. Hsueh et al., A MICROFABRICATED, ELECTROCHEMILUMINESCENCE CELL FOR THE DETECTION OFAMPLIFIED DNA, Sensors and actuators. B, Chemical, 33(1-3), 1996, pp. 110-114
Citations number
5
Categorie Soggetti
Electrochemistry,"Chemistry Analytical","Instument & Instrumentation
Journal title
Sensors and actuators. B, Chemical → ACNP
ISSN journal
09254005
Volume
33
Issue
1-3
Year of publication
1996
Pages
110 - 114
Database
ISI
SICI code
0925-4005(1996)33:1-3<110:AMECFT>2.0.ZU;2-0
Abstract
An electrochemical cell for the generation and detection of electroche miluminescence of tris (2,2'-bipyridyl) ruthenium(II) has been microfa bricated in silicon. The cell has been designed for use in the detecti on and quantification of DNA which has been amplified by the polymeras e chain reaction. The cell is a vertical assembly of micromachined sil icon and glass substrates, containing a gold, thin film cathode and an indium tin oxide, thin film anode. The anode is transparent, enabling the detection of the luminescence by an external photodetector. Preli minary tests have yielded the detection of free TBR concentrations fro m 10(-9) M to 10(-3) M with a cell volume of 85 mu l.