CELLULAR AND SUBCELLULAR-LOCALIZATION OF GLYCOGENIN IN CHICKEN RETINA

We investigated the cellular and subcellular localization of glycogeni n in chicken retina using a polyclonal antibody raised against chicken muscle glycogenin. The antiserum recognized both free and glycogen-bo und glycogenin on dot blots. Immunocytochemistry revealed an uniform s taining of all the retina layers except for the outer segments and gan glion cells layers that were very weakly stained and for the inner seg ments layer and a zone between the inner nuclear and inner plexiform l ayers that were heavily stained. Electron microscopy of neuronal cells showed immunoreactivity localized in the cytoplasm and nucleus. This is the first description of the cellular and subcellular localization of glycogenin. Our results suggest that the biosynthesis of glycogen c ould begin in both, the cytoplasm and nucleus of the neurone.