Y. Wada et al., ELECTROPHORETIC GELS OF DENTIN MATRIX PROTEINS AS DIFFUSION MEDIA FORIN-VITRO MINERALIZATION, Journal of dental research, 75(6), 1996, pp. 1381-1387
Non-collagenous proteins of dentin and bone have important-effects on
mineralization which have been studied by various in vitro systems. We
developed an in vitro mineralization system using electrophoretic gel
s as diffusion media of calcium and phosphate ions. Calcium and phosph
ate ions were diffused naturally or propelled by electric potential. C
alcium phosphate was precipitated in the gel, and the precipitation wa
s affected by proteins in the gel which had been separated by electrop
horesis. We applied this system to analysis of non-collagenous protein
s of dentin. Among the proteins, phosphophoryns promoted calcium phosp
hate precipitation in the natural-diffusion system. A non-collagenous
protein having a molecular mass of 60 kDa inhibited precipitation. The
results were different, however, in the electric-diffusion system, in
which phosphophoryns had a negative effect. The present system enable
d us to compare the effects of plural proteins rapidly, even using unp
urified material.