ROLE OF EXTENDED CULTURE TIME ON SYNERGY OF FLUCONAZOLE AND HUMAN MONOCYTE-DERIVED MACROPHAGES IN CLEARING CANDIDA-ALBICANS

Possible enhanced candidacidal synergy of fluconazole and human monocy te-derived macrophages by extended culture time was studied, Synergist ic candidacidal activity increased from 1 day io 3 days and was maxima l at 6 days. Enhanced synergistic candidacidal activity at 144 h was o bserved over a 10-fold challenge dose range. Enhanced synergy with cul ture time was seen with fluconazole-susceptible and -resistant isolate s of Candida albicans. These findings emphasize the importance of dosa ge and an adequate period of therapy.