EFFECT OF SURFACTANTS ON CELLULASE PRODUCTION BY NECTRIA-CATALINENSIS

Authors
Citation
Ag. Pardo, EFFECT OF SURFACTANTS ON CELLULASE PRODUCTION BY NECTRIA-CATALINENSIS, Current microbiology, 33(4), 1996, pp. 275-278
Citations number
24
Categorie Soggetti
Microbiology
Journal title
ISSN journal
03438651
Volume
33
Issue
4
Year of publication
1996
Pages
275 - 278
Database
ISI
SICI code
0343-8651(1996)33:4<275:EOSOCP>2.0.ZU;2-W
Abstract
The effect of different nonionic surfactants (Tween 80, Tween 20, Trit on X-100) and polyethylene glycol (PEG 6000) was tested on cellulolyti c enzyme system production. Tween 80 gave the highest yield of endoglu canase, exoglucanase, and cellobiase at the 20th day of growth, presum ably by causing increased permeability of cell membranes and/or by pro moting the release of cell-bound enzymes. Maximal yield of endoglucana se was achieved with 1.7 mM Tween 80, whereas exoglucanase and cellobi ase were at 0.85 mM. In the same way, this compound increased fungal g rowth. On the other hand, Tween 20 and Triton X-100 inhibited growth a nd cellulolytic enzyme production. High yields of endoglucanase and ex oglucanase were achieved with PEG 6000 in comparison with the control, presumably by increasing enzyme stability.