Jr. Petersen et al., LASER-INDUCED RESONANCE ENERGY-TRANSFER - A NOVEL-APPROACH TOWARDS ACHIEVING HIGH-SENSITIVITY IN CAPILLARY ELECTROPHORESIS .1. CLINICAL DIAGNOSTIC APPLICATION, Journal of chromatography, 744(1-2), 1996, pp. 37-44
Citations number
29
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Most of the procedures currently performed by capillary zone electroph
oresis (CZE) with laser induced fluorescence detection requires prior
derivatization. This increases cost, the turn-around-time and chances
of extraneous contaminations. CZE with laser induced resonance energy
transfer is demonstrated as a viable alternative for detecting non-flu
orescent compounds with no prior derivatization. The feasibility of th
is approach is demonstrated by separating and directly detecting salic
ylic acid (2,4-dihydroxybenzoic acid), gentisic acid (o-methoxybenzoic
acid), salicyluric acid (o-hydroxyhippuric acid) and 4-aminosalicylic
acid in urine. The detection of salicylate in serum is also shown. Th
e method is highly sensitive with detection limits in the 1 . 10(-7) M
range. Importantly it requires no prior preconcentration or sample pr
eparation and can be used with complex sample matrices such as serum a
nd urine.