Sample load is in proportion to the square of capillary inner diameter
in capillary electrophoresis (CE). With wide-bore capillaries, single
run fraction collection of CE often gives enough material for further
identification techniques, such as matrix-assisted laser desorption i
onization time-of-flight (MALDI-TOF) MS and peptide sequencing. Limita
tions of using wide bore capillaries for CE and solutions to them are
discussed in this investigation. An existing CE instrument has been mo
dified in order to use wide-bore capillaries. Tryptic digest peptides
have been identified with off-line wide bore CE-MALDI-TOF-MS technique
s.