SELECTIVITY, DIFFERENTIAL MOBILITY AND RESOLUTION AS PARAMETERS TO OPTIMIZE CAPILLARY ELECTROPHORETIC SEPARATION

Selectivity, differential mobility, and resolution have been tested as the optimization functions to find the optimum pH of operational elec trolyte for separation by capillary electrophoresis when organic acids occurring in human serum have been selected as a model mixture for co mputer simulations. Using tabulated values of ionic mobilities and pK( a) values, either selectivity or differential mobility or resolution f or the hardest-to-separate pair of sepasands are calculated and plotte d vs. pH. The optimum pH is the pH value, at which the optimization fu nction reaches its maximum.