PURIFICATION OF THE MONOCLONAL HEPARIN ANTIBODY H-1.18

An antibody of the (immunoglobulin) IgG(1) subclass against heparin wa s purified. Here we report on the purification of the heparin antibody . Ammonium sulfate precipitation was performed and showed a high purit y of the precipitate. In the heparin radioimmunoassay it showed a high heparin binding. Capillary electrophoresis showed that albumin and ot her proteins were separated from the heparin antibody. The purificatio n method allowed a large scale production of the heparin antibody.