HUMAN-CHROMOSOME-3 - HIGH-RESOLUTION FLUORESCENCE IN-SITU HYBRIDIZATION MAPPING OF 40 UNIQUE NOTI LINKING CLONES HOMOLOGOUS TO GENES AND CDNAS

Forty new Notl linking clones representing sequence tagged sites (STSs ) were mapped by fluorescence in site hybridization (FISH) to differen t regions of human chromosome 3 (HSA3). Clone NL1-245, containing huma n aminoacylase 1, was localized to 3p21.2-p21.1. Our previous localiza tion of the CLC-2 chloride channel protein gene was refined to 3q27. C lone NL2-316 most likely contains a translocon-associated protein gamm a-subunit gene and was mapped to 3q23-q24. To our knowledge, this is t he first time this gene has been mapped. One Notl linking clone (NL1-2 29) probably contains a new protein phosphatase gene. This clone was m apped to 3p25. Five Notl linking clones probably contain human express ed sequence tags (ESTs), as they possess sequences with a high level o f identity (> 90%) to cDNA clones. Other clones show 56-85% homology t o known mammalian and human genes with various functions, including on cogenes and tumour-suppressor genes. These clones might represent new genes.