COMBINED EXPRESSION OF ASPERGILLUS-NIDULANS ENDOXYLANASE X24 AND ASPERGILLUS-ORYZAE ALPHA-AMYLASE IN INDUSTRIAL BAKERS YEASTS AND THEIR USEIN BREAD-MAKING

Authors
MONFORT A BLASCO A PRIETO JA SANZ P
Citation
A. Monfort et al., COMBINED EXPRESSION OF ASPERGILLUS-NIDULANS ENDOXYLANASE X24 AND ASPERGILLUS-ORYZAE ALPHA-AMYLASE IN INDUSTRIAL BAKERS YEASTS AND THEIR USEIN BREAD-MAKING, Applied and environmental microbiology, 62(10), 1996, pp. 3712-3715
Citations number
21
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
62
Issue
10
Year of publication
1996
Pages
3712 - 3715
Database
ISI
SICI code
0099-2240(1996)62:10<3712:CEOAEX>2.0.ZU;2-M
Abstract
The Aspergillus nidulans endoxylanase X24 and the Aspergillus oryzae a lpha-amylase cDNAs were placed under the control of the Saccharomyces cerevisiae actin promoter (pACT1) and introduced into baker's yeast. B read made with transformants expressing both enzymes (YEpACT-AMY-ACT-X 24) showed a 30% increase in volume and reduced firmness in comparison with that produced with a commercial strain. Endoxylanase X24 and alp ha-amylase seem to act synergistically to improve the quality of bread in terms of volume and density.