EVALUATION OF CYCLOHEXENOESCULETIN-BETA-D-GALACTOSIDE AND 8-HYDROXYQUINOLINE-BETA-D-GALACTOSIDE AS SUBSTRATES FOR THE DETECTION OF BETA-GALACTOSIDASE

Authors
JAMES AL PERRY JD FORD M ARMSTRONG L GOULD FK
Citation
Al. James et al., EVALUATION OF CYCLOHEXENOESCULETIN-BETA-D-GALACTOSIDE AND 8-HYDROXYQUINOLINE-BETA-D-GALACTOSIDE AS SUBSTRATES FOR THE DETECTION OF BETA-GALACTOSIDASE, Applied and environmental microbiology, 62(10), 1996, pp. 3868-3870
Citations number
9
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
62
Issue
10
Year of publication
1996
Pages
3868 - 3870
Database
ISI
SICI code
0099-2240(1996)62:10<3868:EOCA8>2.0.ZU;2-L
Abstract
We describe the synthesis of two new substrates for the detection of b eta-galactosidase and evaluate their performance in comparison with th at of -bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal). Of 1 71 Enterobacteriaceae strains that were able to hydrolyze X-Gal, 166 ( 97.1%) also hydrolyzed cyclohesenocsculetin-beta-D-galactoside whereas only 96 (56.1%) showed evidence of hydrolysis of 8-hydroxyquinoline-b eta-D-galactoside. No false-positive results were observed with either substrate.