A GLYCEROL-3-PHOSPHATE DEHYDROGENASE-DEFICIENT MUTANT OF SACCHAROMYCES-CEREVISIAE EXPRESSING THE HETEROLOGOUS XYL1 GENE

Authors
LIDEN G WALFRIDSSON M ANSELL R ANDERLUND M ADLER L HAHNHAGERDAL B
Citation
G. Liden et al., A GLYCEROL-3-PHOSPHATE DEHYDROGENASE-DEFICIENT MUTANT OF SACCHAROMYCES-CEREVISIAE EXPRESSING THE HETEROLOGOUS XYL1 GENE, Applied and environmental microbiology, 62(10), 1996, pp. 3894-3896
Citations number
24
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
62
Issue
10
Year of publication
1996
Pages
3894 - 3896
Database
ISI
SICI code
0099-2240(1996)62:10<3894:AGDMOS>2.0.ZU;2-8
Abstract
The gene XYLI, encoding a xylose reductase, from Pichia stipitis was t ransformed into a mutant of Saccharomyces cerevisiae incapable of glyc erol production because of deletion of the genes GPD1 and GPD2. The tr ansformed strain was capable of anaerobic glucose conversion in the pr esence of added xylose, indicating that the xylose reductase reaction can fulfill the role of the glycerol-3-phosphate dehydrogenase reactio n as a redox sink. The specific xylitol production rate obtained was 0 .38 g g(-1) h(-1).