RAPID EXTRACTION OF DNA AND RIBOSOMAL-RNA FROM SEDIMENTS BY A NOVEL HYDROXYAPATITE SPIN-COLUMN METHOD

Authors
PURDY KJ EMBLEY TM TAKII S NEDWELL DB
Citation
Kj. Purdy et al., RAPID EXTRACTION OF DNA AND RIBOSOMAL-RNA FROM SEDIMENTS BY A NOVEL HYDROXYAPATITE SPIN-COLUMN METHOD, Applied and environmental microbiology, 62(10), 1996, pp. 3905-3907
Citations number
20
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
62
Issue
10
Year of publication
1996
Pages
3905 - 3907
Database
ISI
SICI code
0099-2240(1996)62:10<3905:REODAR>2.0.ZU;2-I
Abstract
We describe a novel hydroxyapatite spin-column method of nucleic acid extraction from natural sediments by which DNA and rRNA can be extract ed separately. This very rapid method produces pure nucleic acid that can be utilized in some of the most common molecular biological proced ures used in the analysis of natural microbial communities.