BROMODEOXYURIDINE - A DIAGNOSTIC-TOOL IN BIOLOGY AND MEDICINE .3. PROLIFERATION IN NORMAL, INJURED AND DISEASED TISSUE, GROWTH-FACTORS, DIFFERENTIATION, DNA-REPLICATION SITES AND IN-SITU HYBRIDIZATION

This paper is a continuation of parts I (history, methods and cell kin etics) and II (clinical applications and carcinogenesis) published pre viously (Dolbeare, 1995 Histochem. J. 27, 339, 923). Incorporation of bromodeoxyuridine (BrdUrd) into DNA is used to measure proliferation i n normal, diseased and injured tissue and to follow the effect of grow th factors. Immunochemical detection-of BrdUrd can be used to determin e proliferative characteristics of differentiating tissues and to obta in birth dates for actual differentiation events. Studies are also des cribed in which BrdUrd is used to follow the order of DNA replication in specific chromosomes, DNA replication sites in the nucleus and to m onitor DNA repair. BrdUrd incorporation has been used as a tool for in situ hybridization experiments.