The aim of this study was to compare three different test methods for
assaying the biodegradability of starch-based materials. The materials
tested included some commercial starch-based materials and thermoplas
tic starch film prepared by extrusion from native potato starch and gl
ycerol. Enzymatic hydrolysis was performed using excess Bacillus liche
niformis alpha-amylase and Aspergillus niger glucoamylase at 37 degree
s C. The degree of degradation was assayed by measuring the dissolved
carbohydrates and the weight loss of the samples. The head-space test
was based on carbon dioxide evolution using sewage sludge as an inocul
um. The composting experiments were carried out in an insulated commer
cial composter bin. The degradation was evaluated visually at weekly i
ntervals, and the weight loss of the samples was measured after compos
ting. Good correlation was found among the three different test method
s.