IMMUNOASSAYS IN SOL-GEL MATRICES

Citation
J. Livage et al., IMMUNOASSAYS IN SOL-GEL MATRICES, JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY, 7(1-2), 1996, pp. 45-51
Citations number
29
Categorie Soggetti
Material Science
ISSN journal
09280707
Volume
7
Issue
1-2
Year of publication
1996
Pages
45 - 51
Database
ISI
SICI code
0928-0707(1996)7:1-2<45:IISM>2.0.ZU;2-N
Abstract
Parasites have been encapsulated within sol-gel silica matrices. Therm oporometry measurements show that the pore size depends strongly on ex perimental conditions. Pores as large as 100 Angstrom in diameter can be obtained, allowing the diffusion of large biomolecules such as immu noglobulins. TEM observations, performed on procaryote (bacteria) and eucaryote (protozoa) specimen show that the cellular organization and the integrity of the plasma membrane of entrapped parasites are preser ved. Moreover they retain their antigenic activity and can react speci fically with the corresponding antibodies. Sol-gel matrices have then been used for the realization of enzyme-linked immunosorbent assays (E LISA) directly with the sera of infected patients. Two examples are de scribed, one with parasitic protozoa, Leishmania, and the other one wi th the cystic hydatid stage of tapeworm parasites, Echinococcus granul osus.