Y. Yufu et al., EXPRESSION OF EVL1 AND THE RETINOBLASTOMA GENES IN ACUTE MYELOGENOUS LEUKEMIA WITH T(3-13)(Q26-Q13-14), American journal of hematology, 53(1), 1996, pp. 30-34
The EVI1 DNA-binding protein gene on chromosome 3q26 has been reported
to be activated in some leukemia cells with alterations in 3q26, We p
resent an acute myelogenous leukemia (AML) patient with a rare chromos
omal translocation, t(3;13)(q26,2;q13-14), By reverse transcription- p
olymerase chain reaction, we detected active transcription of the EVI1
gene in the patient's leukemia cells, The retinoblastoma susceptibili
ty (Rb) gene, a tumor-suppressor gene, is located at chromosome 13q14
and is within the other translocation breakpoint in this patient, The
expression of the Rb gene product was found to be substantially decrea
sed in the patient's leukemia cells by Western blotting, Southern blot
analysis, however, revealed no gross abnormalities of the Rb gene. Al
though it is unlikely that the Rb gene is directly involved in this tr
anslocation, the loss of the Rb gene product combined with the activat
ion of the EVI1 gene may have led to the development of leukemia. (C)
1996 Wiley-Liss, Inc.