GENETIC AND CONTIG MAP OF A 2200-KB REGION ENCOMPASSING 5.5 CM ON CHROMOSOME-1 OF ARABIDOPSIS-THALIANA

In the course of the isolation of the MONOPTEROS (MP) gene, required f or primary root formation in Arabidopsis thaliana, a yeast artificial chromosome (YAC) contig encompassing approximately 2200 kilobases corr esponding to 5.5 cM on the top arm of chromosome 1 was established. Fo rty-six YAC clones were characterized and 12 new restriction fragment length polymorphism (RFLP) markers are presented. Three new codominant amplified polymorphic sequence (CAPS) markers were generated that ena bled high resolution genetic mapping and correlation of physical and g enetic distances along the contig. The map contributes to the completi on of a physical map of the Arabidopsis genome and should facilitate p ositional cloning of other genes in the region as well as studies on g enome organization. We also present another set of 11 physically linke d probes, as well as mapping data for additional RFLP markers within a broader interval of 10.4 cM.