C. Foucaud et al., DEVELOPMENT OF A CHEMICALLY-DEFINED MEDIUM FOR THE GROWTH OF LEUCONOSTOC-MESENTEROIDES, Applied and environmental microbiology, 63(1), 1997, pp. 301-304
A chemically defined medium for the growth of Leuconostoc mesenteroide
s was developed. This medium contained lactose, Mn2+, Mg2+, 12 amino a
cids, eight vitamins, adenine, uracil, and Tween 80. We showed the ben
eficial effect of aerobic conditions on growth and that potassium phos
phate (135 mM) is a suitable buffer. The growth rate in this medium wa
s 0.85 +/- 0.10 h(-1) for the six strains examined, and cell densities
up to 3.5 x 10(9) CFU/ml were attained.