RAPID AND EFFICIENT PURIFICATION METHOD FOR SMALL, HYDROPHOBIC, CATIONIC BACTERIOCINS - PURIFICATION OF LACTOCOCCIN-B AND PEDIOCIN PA-1

Authors
VENEMA K CHIKINDAS ML SEEGERS JFML HAANDRIKMAN AJ LEENHOUTS KJ VENEMA G KOK J
Citation
K. Venema et al., RAPID AND EFFICIENT PURIFICATION METHOD FOR SMALL, HYDROPHOBIC, CATIONIC BACTERIOCINS - PURIFICATION OF LACTOCOCCIN-B AND PEDIOCIN PA-1, Applied and environmental microbiology, 63(1), 1997, pp. 305-309
Citations number
23
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
63
Issue
1
Year of publication
1997
Pages
305 - 309
Database
ISI
SICI code
0099-2240(1997)63:1<305:RAEPMF>2.0.ZU;2-4
Abstract
The bacteriocins lactococcin B and pediocin PA 1 were purified by etha nol precipitation, preparative isoelectric focusing, and ultrafiltrati on. The procedure reproducibly leads to high final yields in compariso n to the generally low yields obtained by column chromatography. Speci fically, during isoelectric focusing no loss of activity occurs. The m ethod, in general, should be applicable to small, hydrophobic, cationi c bacteriocins.