Jm. Robinson et al., A METHOD FOR COLOCALIZATION OF TUBULAR LYSOSOMES AND MICROTUBULES IN MACROPHAGES - FLUORESCENCE MICROSCOPY OF INDIVIDUAL CELLS, The Journal of histochemistry and cytochemistry, 44(10), 1996, pp. 1109-1114
Routinely used procedures for chemical fixation often fail to preserve
delicate membrane-bounded tubular structures in a variety of cell typ
es. Fixation procedures commonly employed in immunocytochemical studie
s for localization of structural proteins, such as those found in cyto
skeletal elements, may also degrade these tubular structures. Here we
describe a proedure that preserves the elaborate tubular lysosome syst
em found in stimulated macrophages and allows the subsequent immunoflu
orescence localization of microtubules in the same cells. Use of this
methodology permits the assessment of the spatial relationship between
tubular lysosomes and microtubules in macrophages.