M. Stankiewicz et al., 3'-NONCODING SEQUENCES OF THE CTA-1 GENE ENHANCE EXPRESSION OF THE RECOMBINANT SERINE-PROTEASE INHIBITOR, CPTI-II, IN SACCHAROMYCES-CEREVISIAE, Acta Biochimica Polonica, 43(3), 1996, pp. 525-529
Expression of the gene coding for the recombinant trypsin inhibitor, C
PTI II, was enhanced tenfold when yeast transcription terminating sequ
ences were added to the expression cassette of the pJK6 yeast vector.
The yield was further increased about 20% in the BJ5464 yeast strain,
defective in vacuolar proteases.