Ac. Rovescalli et al., CLONING AND CHARACTERIZATION OF 4 MURINE HOMEOBOX GENES, Proceedings of the National Academy of Sciences of the United Statesof America, 93(20), 1996, pp. 10691-10696
Four novel murine homeobox genes, Uncx-4.1, OG-2, OG-9, and OG-12, wer
e cloned and partially sequenced, The amino acid sequence of the mouse
Uncx-4.1 homeodomain is closely related to the sequence of the unc-4
homeodomain of Caenorhabditis elegans, However, the OG-2, OG-9, and OG
-12 homeodomains are relatively diverged and are not closely related t
o any previously described homeodomain, Northern blot analyses reveale
d multiple bands of Uncx-4.1, OG-2, OG-9, and OG-12 poly(A)(+) RNA in
RNA from mouse embryos and adults that change during development and s
howed that each gene is expressed in a tissue-specific manner, OG-12 c
DNAs were cloned that correspond to two alternatively spliced species
of OG-12 mRNA, Three major bands of Uncx-4.1 poly(A)(+) RNA were found
only in RNA from adult mouse brain, but an additional band was observ
ed in RNA from all of the other tissues tested, Major bands of OG-9 an
d OG-2 poly(A)(+) RNA were found only in RNA from striated muscle; how
ever, trace bands were detected in RNA from other tissues.