AGE-RELATED PATTERNS OF LECTIN-BINDING NUCLEAR GLYCOPROTEINS OF HAMSTER LIVER

We studied the age-related patterns of lectin-binding liver nuclear gl ycoproteins from hamsters between 5 and 35 weeks of age. The examinati ons of the carbohydrate structures of liver nuclear glycoproteins in r elation to the age of hamsters were carried out after electrophoresis and blotting by a very sensitive immunological detection system with h ighly specific digoxigenin-labelled lectins. The results reported in t he present study do not show any significant changes in the patterns o f nuclear glycoproteins with regards to the age of hamsters except the decrease in affinity of wheat germ agglutinin (WGA) to 63 kDa glycopr otein bearing single O-linked N-acetylglucosamine residues.