Sulphur deficiency has become increasingly widespread in wheat in the
U.K. Growth, nutrient content and biochemical responses to S and N sup
ply of a breadmaking wheat variety (Hereward) and a non-breadmaking va
riety (Riband) were investigated in a pot experiment. Shoot dry matter
(DM) at stem extension (Zadok's GS 37) and at maturity was increased
markedly by S. Grain production of the Riband variety was more suscept
ible to the imbalance of N to S than the Hereward variety. At GS 37, t
he concentrations of total S and sulphate-S of shoots, chlorophyll met
er readings and the concentrations of glutathione of the uppermost ful
ly expanded leaves were increased significantly by increasing S supply
, whereas the concentrations of nitrate and amides were decreased by S
. The greatest relative changes in response to S supply were those of
the glutathione and asparagine concentrations. Riband also showed grea
ter response to S than Hereward. Critical values of various diagnostic
indices at GS 37 were derived from the relationships between DM yield
and different indices. The two varieties showed similar diagnostic cu
rves except that for the ratio of total N to total S (N:S) in shoots.
Either total S or sulphate-S can be used alone as a good indicator of
deficiency, and with values of 1500 and 190 mg kg(-1) DM in shoots for
the two indices respectively. There was also a well defined relations
hip between DM yield and the glutathione concentration, with a critica
l value of 240 nmol g(-1) FW. There were no advantages of using % of t
otal S as sulphate-S. Shoot N:S ratio was found to be less accurate in
predicting S deficiency than total S or sulphate-S. For prognostic pu
rposes, a much higher S status at GS 37 was required to ensure no loss
es of DM yield due to S deficiency at maturity.