UNFOLDING MECHANISM AND STABILITY OF IMMOBILIZED PAPAIN

Authors
RIALDI G BATTISTEL E
Citation
G. Rialdi et E. Battistel, UNFOLDING MECHANISM AND STABILITY OF IMMOBILIZED PAPAIN, Journal of thermal analysis, 47(1), 1996, pp. 17-25
Citations number
17
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of thermal analysis → ACNP
ISSN journal
03684466
Volume
47
Issue
1
Year of publication
1996
Pages
17 - 25
Database
ISI
SICI code
0368-4466(1996)47:1<17:UMASOI>2.0.ZU;2-D
Abstract
The thermal stability of papain in free solution or immobilized on CPC -silica has been investigated by DSC. At neutral pH, in both condition s, the protein undergoes a thermal transition which corresponds to the sum of two transitions associated with the unfolding of the two domai ns of the protein. At low pH, in the case of immobilized papain, only one transition is observed.