EFFECT OF TRAY AMBER MUTATIONS ON F-PLASMID TRAY PROMOTER ACTIVITY IN-VIVO

Authors
SILVERMAN PM SHOLL A
Citation
Pm. Silverman et A. Sholl, EFFECT OF TRAY AMBER MUTATIONS ON F-PLASMID TRAY PROMOTER ACTIVITY IN-VIVO, Journal of bacteriology, 178(19), 1996, pp. 5787-5789
Citations number
29
Categorie Soggetti
Microbiology
Journal title
Journal of bacteriology → ACNP
ISSN journal
00219193
Volume
178
Issue
19
Year of publication
1996
Pages
5787 - 5789
Database
ISI
SICI code
0021-9193(1996)178:19<5787:EOTAMO>2.0.ZU;2-3
Abstract
We have examined the effect of the F plasmid TraY protein on tm gene e xpression in vivo. Expression was assayed as alkaline phosphatase acti vity in cells containing a traY Phi(traA'-'phoA)hyb operon under traY promoter control. Amber mutations in traY significantly reduced alkali ne phosphatase activity. Since nonsense polarity effects were minimal, if they occurred at all, these data provide the first direct evidence that TraY regulates tra gene expression.