IMPLICATION OF ILE-69 AND THR-182 RESIDUES IN KINETIC CHARACTERISTICSOF IRT-3 (TEM-32) BETA-LACTAMASE

Authors
FARZANEH S CHAIBI EB PEDUZZI J BARTHELEMY M LABIA R BLAZQUEZ J BAQUERO F
Citation
S. Farzaneh et al., IMPLICATION OF ILE-69 AND THR-182 RESIDUES IN KINETIC CHARACTERISTICSOF IRT-3 (TEM-32) BETA-LACTAMASE, Antimicrobial agents and chemotherapy, 40(10), 1996, pp. 2434-2436
Citations number
26
Categorie Soggetti
Pharmacology & Pharmacy",Microbiology
Journal title
Antimicrobial agents and chemotherapy → ACNP
ISSN journal
00664804
Volume
40
Issue
10
Year of publication
1996
Pages
2434 - 2436
Database
ISI
SICI code
0066-4804(1996)40:10<2434:IOIATR>2.0.ZU;2-9
Abstract
The substitution of a methionine for an isoleucine at position 69 (Met 69IIe), which causes inhibitor resistance to TEM-type beta-lactamases (IRT-3 and IRT-I69), altered the positions of the Asn-170 and Glu-166 side chains as well as the position of the catalytic water molecule. A novel hydrogen bond between the hydroxyl of Thr-182 and the carbonyl of Glu-64 was expected to be responsible for the increase in the catal ytic activity of the IST-T182 and IRT-3 enzymes compared with those of TEM-1 and IRT-I69, respectively.