DETERMINATION OF CHLOROPHYLLS AND CAROTENOIDS OF MARINE-PHYTOPLANKTON- SEPARATION OF CHLOROPHYLL-A FROM DIVINYL-CHLOROPHYLL A AND ZEAXANTHIN FROM LUTEIN
F. Vidussi et al., DETERMINATION OF CHLOROPHYLLS AND CAROTENOIDS OF MARINE-PHYTOPLANKTON- SEPARATION OF CHLOROPHYLL-A FROM DIVINYL-CHLOROPHYLL A AND ZEAXANTHIN FROM LUTEIN, Journal of plankton research, 18(12), 1996, pp. 2377-2382
A rapid reverse-phase HPLC method is presented for the identification
and quantification of most of the phytoplankton pigments. This method
yields the resolution of divinyl-chlorophyll a and chlorophyll a, as w
ell as the partial resolution of lutein and zeaxanthin, and of divinyl
-chlorophyll b and chlorophyll b. In addition, chlorophylls c(1,2) and
c(3) are well resolved. The analysis time for one sample is 20 min, w
hich makes this method particularly suited when large numbers of sampl
es have to be processed.