RAPID PREPARATION OF BACTERIAL-DNA FOR PULSED-FIELD GEL-ELECTROPHORESIS

Authors
MATUSHEK MG BONTEN MJM HAYDEN MK
Citation
Mg. Matushek et al., RAPID PREPARATION OF BACTERIAL-DNA FOR PULSED-FIELD GEL-ELECTROPHORESIS, Journal of clinical microbiology, 34(10), 1996, pp. 2598-2600
Citations number
8
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
34
Issue
10
Year of publication
1996
Pages
2598 - 2600
Database
ISI
SICI code
0095-1137(1996)34:10<2598:RPOBFP>2.0.ZU;2-P
Abstract
A disadvantage of genotyping bacterial strains by pulsed-field gel ele ctrophoresis is that the procedure requires up to 6 days to complete. We modified a standard pulsed-field gel electrophoresis method (B. E. Murray, K. V, Singh, J. D. Heath, B. R. Sharma, and G. M. Weinstock, J , Clin, Microbiol. 28:2059-2063, 1990) so that it could be completed i n less than 3 days. We successfully applied this method to the analysi s of a variety of gram-positive and gram-negative bacteria.