SOMATIC EMBRYOGENESIS AND ESCULIN FORMATION IN CALLI AND EMBRYOIDS FROM BARK EXPLANTS OF AESCULUS-HIPPOCASTANUM L

Authors
GASTALDO P CAVIGLIA AM CARLI S PROFUMO P
Citation
P. Gastaldo et al., SOMATIC EMBRYOGENESIS AND ESCULIN FORMATION IN CALLI AND EMBRYOIDS FROM BARK EXPLANTS OF AESCULUS-HIPPOCASTANUM L, PLANT SCI, 119(1-2), 1996, pp. 157-162
Citations number
7
Categorie Soggetti
Plant Sciences
Journal title
PLANT SCIENCE
ISSN journal
01689452 → ACNP
Volume
119
Issue
1-2
Year of publication
1996
Pages
157 - 162
Database
ISI
SICI code
0168-9452(1996)119:1-2<157:SEAEFI>2.0.ZU;2-W
Abstract
Somatic embryogenesis was obtained from bark explants of Aesculus hipp ocastanum L. cultured in agarized Murashige and Skoog medium supplemen ted with some growth substances (2,4-dichlorophenoxyacetic acid, kinet ine, I-naphthalene acetic acid, 2.0 mg/l of each). Esculin and its agl ucon, esculetin, were tested in calli and embryoids by HPLC; their pre sence in somatic embryos was confirmed by H-1-NMR. The results indicat e that esculin and esculetin were present in noticeable amounts in all of the analyzed samples.