Simultaneous enhanced biological phosphate uptake and biological denit
rification under anoxic conditions were investigated in a modified lab
-scale nutrient removal activated sludge system (DEPHANOX). The aim of
the experiments was to find whether it can be technologically covenie
nt to use the capability of poly-P bacteria of taking up phosphate und
er anoxic condition by utilizing nitrate as electron acceptor. An expe
rimental comparison of removal efficiencies with a more conventional t
reatment flow scheme (JHB), using low influent TKN/COD ratio, was also
carried out. Phosphate uptake in anoxic condition was compared to tha
t in oxic environment in batch tests. PHB anoxic and oxic degradation
was evaluated by respirometric tests. Results of the long-term operati
on of continuous-flow lab-scale system as well as results of batch tes
ts showed that the anoxic phosphate uptake with simultaneous denitrifi
cation after a previous anaerobic substrate uptake could significantly
reduce the extent of competition for organic substrate between poly-P
bacteria and denitrifiers. A side-stream nitrification in fixed-film
reactor reduced the losses of organic carbon by oxidation and stabiliz
ed the slow-growing population of nitrifiers in the system. Copyright
(C) 1996 IAWQ.