Microbial removal of nitrate from drinking water was studied in labora
tory columns packed with unprocessed short fiber (low quality) cotton
(Gossypium hirsutum). Cotton served as the sole chemical and physical
substrate for the microbial population. Removal of nitrate was rapidly
achieved without the formation of nitrite. Cotton (cellulose)-depende
nt denitrification was affected by changes in temperature: denitrifica
tion rates at 14 degrees C were approximately half of the rates observ
ed at 30 degrees C. The cotton was entirely consumed in the process. I
n a fresh reactor, colonization of substrate by bacteria appears to be
the rate limiting factor in the removal of nitrate. Copyright (C) 199
6 IAWQ.