CAPILLARY ELECTROPHORETIC DETERMINATION OF THE CONSTITUENTS OF PAEONIAE RADIX

A method combining the techniques of capillary zone electrophoresis (C ZE) and micellar electrokinetic chromatography (MEKC) has been develop ed to separate a total of eight peony constituents. The CZE method was used to determine the content of paeonol, oxypaeoniflorin, benzoic ac id, pentagalloylglucose and gallic acid, and MEKC technique based on s odium cholate was applied to analyze albiflorin, paeoniflorin, benzoyl albiflorin, paeonol and oxypaeoniflorin, Linearity around two orders o f magnitude of concentration was generally obtained and limits of dete ction for these compounds were in the range of 2.6-23.7 mu g/ml. The r elative standard deviations of migration times were less than 1.43% (n =6). Contents of peony constituents in an ethanol-water extract of Pae onia lactiflora Pall. sample could easily be determined by this method .