EVALUATION OF P53 PROTEIN EXPRESSION IN RENAL-CELL CARCINOMA - COMPARISON OF 2 ANTIBODIES

The aims of this study were to establish a profile for the expression of p53 in primary renal cell carcinoma using the polyclonal antibody N CL-CB1 and the monoclonal antibody D07, and to compare the results of staining with both antibodies. Ninety-six cases were studied using for malin-fixed paraffin-embedded tissue. Positive nuclear staining ranged from 5% (D07) to 12% (NCL-CM1). Positive cytoplasmic staining ranged from 7% (D07) to 25% (NCL-CM1) of cases. Interobserver agreement was e xcellent. The findings suggest that such a low level of immunohistoche mical positivity reduces anp potential prognostic value for p53 in thi s tumour type.