T. Pelet et al., RESCUE OF SENDAI VIRUS CDNA TEMPLATES WITH CDNA CLONES EXPRESSING PARAINFLUENZA VIRUS TYPE-3 N, P AND L PROTEINS, Journal of General Virology, 77, 1996, pp. 2465-2469
Several years ago, we reported that a Sendai virus (SeV) defective gen
ome (DIH4(UV)) could be rescued in vivo with human parainfluenza virus
type 1 (hPIV1) and bovine PIV3 but not by measles virus or vesicular
stomatitis virus. It was concluded that the cis-acting RNA sequences w
ere conserved within the SeV/PIV1/PIV3 group but that interactions bet
ween the polymerase complex (P-L) and the template protein N were uniq
ue for each virus. We have re-examined these conclusions using protein
s expressed from cloned N, P and L genes for SeV and PIV3. The results
demonstrate the specificity of the protein-protein interactions betwe
en polymerase and template, and confirm the prediction of the earlier
work that PIV3 N, P and L proteins are capable of assembling and repli
cating SeV mini-genomes also expressed from a cDNA clone.