GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR PROMOTES PROLONGED SURVIVAL AND THE SUPPORT OF VIRULENT INFECTION BY AFRICAN SWINE FEVER VIRUS OF MACROPHAGES GENERATED FROM PORCINE BONE-MARROW AND BLOOD
S. Denham et al., GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR PROMOTES PROLONGED SURVIVAL AND THE SUPPORT OF VIRULENT INFECTION BY AFRICAN SWINE FEVER VIRUS OF MACROPHAGES GENERATED FROM PORCINE BONE-MARROW AND BLOOD, Journal of General Virology, 77, 1996, pp. 2625-2630
Long-surviving cultures of non-adherent cells of the monocyte-macropha
ge lineage were established from the bone marrow and blood of weanling
pigs by culturing cells from these tissues in the presence of recombi
nant porcine granulocyte-macrophage colony stimulating factor (GM-CSF)
. The cells increased in number, principally during the first 4 weeks
of culture, bound monoclonal antibodies recognizing porcine macrophage
antigens and avidly phagocytosed latex particles. The GM-CSF generate
d mononuclear phagocytes were highly infectable with a virulent Malawi
isolate of African swine fever virus (ASFV) and able to generate leve
ls of virus progeny similar to those produced by fresh ly isolated pig
macrophages. The cultured cells retained their susceptibility to ASFV
infection for as long as the cultures survived i.e. for up to 3 month
s.